Coding

Part:BBa_K2984054:Design

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-PsadIntron-bleRscp-ARS-YFP-SP20-Rbcs2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2051
    Illegal NgoMIV site found at 1607
    Illegal NgoMIV site found at 2528
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Secretion construct for C.reinhardtii designed for transformation in Clamydomnasd and further cloning into Level 2 backbone with MoClo standard.


Source

The construct consist of the sid different Level 0 parts PsadIntron (BBa_K2984046), bleRscp (BBa_K2984045), ARS (BBa_K2984000), YFP (BBa_K2984017), SP20 (BBa_K2984016) and Rbcs2(BBa_K2984018) which were cloned into the level 1 Backbone (BBa_K2984002) using the MoClo standard.


References